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\u751f\u7269\u8d28\u80fd\u6e90\u5df2\u7ecf\u6210\u4e3a\u9886\u5148\u98ce\u80fd\u548c\u592a\u9633\u80fd\u7684\u5168\u7403\u7b2c\u4e00\u5927\u53ef\u518d\u751f\u80fd\u6e90\u54c1\u79cd<\/span><\/b>\uff0c\u523021 <\/span>\u4e16\u7eaa\u4e2d\u53f6\uff0c\u5c06\u5f88\u53ef\u80fd\u8fdb\u4e00\u6b65\u6210\u4e3a\u5168\u7403\u7b2c\u4e00\u5927\u80fd\u6e90\u54c1\u79cd\uff1b\u751f\u7269\u57fa\u6750\u6599<\/span>\u662f\u4e0d\u53ef\u518d\u751f\u5316\u5de5\u539f\u6599\u552f\u4e00\u7684\u66ff\u4ee3\u9014\u5f84\uff0c\u5df2\u6210\u4e3a\u751f\u7269\u8d28\u8f6c\u5316\u7684\u4e3b\u5bfc\u65b9\u5411\u548c\u5316\u5de5\u6750\u6599\u7684\u91cd\u8981\u6765\u6e90\uff1b\u751f\u7269\u8d28\u539f\u6599\u6210\u4e3a\u5168\u7403\u519c\u4e1a\u751f\u4ea7\u7684\u4e3b\u8981\u76ee\u6807\u4e4b\u4e00\u3002\u9ad8\u6548\u4f4e\u6210\u672c\u5229\u7528\u6728\u8d28\u7ea4\u7ef4\u7d20\u539f\u6599\u5df2\u6210\u4e3a\u7814\u7a76\u7684\u70ed\u70b9\u548c\u96be\u70b9\uff0c\u4ee5\u751f\u7269\u8d28\u539f\u6599\u751f\u4ea7\u5316\u5de5\u5e73\u53f0\u5316\u5408\u7269\u662f\u751f\u7269\u8d28\u5de5\u7a0b\u672a\u676520<\/span>\u5e74\u7684\u53d1\u5c55\u91cd\u70b9\u3002\u5173\u952e\u6280\u672f\u4e00\u65e6\u53d6\u5f97\u7a81\u7834\uff0c\u80fd\u6e90\u548c\u6750\u6599\u4e0d\u53ef\u518d\u751f\u7684\u5a01\u80c1\u3001\u751f\u7269\u8d28\u80fd\u6e90\u4e0e\u7cae\u98df\u5b89\u5168\u7684\u77db\u76fe\u3001\u751f\u7269\u8d28\u4ea7\u4e1a\u7684\u89c4\u6a21\u9650\u5236\u3001\u751f\u7269\u8d28\u5de5\u7a0b\u7684\u8f6c\u5316\u6548\u7387\u548c\u80fd\u6e90\u8f6c\u5316\u7387\u7b49\u95ee\u9898\u90fd\u5c06\u8fce\u5203\u800c\u89e3\u3002\u6211\u56fd\u751f\u7269\u8d28\u4ea7\u4e1a\u8d77\u6b65\u665a\uff0c\u7814\u53d1\u6295\u5165\u529b\u5ea6\u5c0f\uff0c\u76f8\u5173\u79d1\u6280\u76f8\u5bf9\u843d\u540e\u3002\u52a0\u5feb\u751f\u7269\u8d28\u98a0\u8986\u6027\u6280\u672f\u7814\u53d1\uff0c\u624d\u80fd\u4f7f\u6211\u56fd\u751f\u7269\u8d28\u4ea7\u4e1a\u8dfb\u8eab\u4e16\u754c\u4e00\u6d41\u884c\u4e1a\uff0c\u652f\u6491\u56fd\u5bb6\u5f3a\u76db\u3002<\/span>\u00a0<\/span><\/p>\n

\uff08\u56db\uff09\u667a\u80fd\u519c\u4e1a\u6280\u672f<\/span><\/p>\n

\u4fe1\u606f\u5316\u662f\u519c\u4e1a\u73b0\u4ee3\u5316\u7684\u5236\u9ad8\u70b9\uff0c\u667a\u80fd\u5316\u662f\u9a71\u52a8\u519c\u4e1a\u73b0\u4ee3\u5316\u7684\u5148\u5bfc\u529b\u91cf<\/span><\/b>\u3002\u667a\u80fd\u6280\u672f\u9a71\u52a8\u7684\u519c\u4e1a\u79d1\u6280\u53d8\u9769\u5bf9\u519c\u4e1a\u53d1\u5c55\u5448\u73b0\u7cfb\u7edf\u6027\u98a0\u8986\u8d8b\u52bf\uff0c\u4ee5\u7269\u8054\u7f51\u3001\u5927\u6570\u636e\u3001\u4eba\u5de5\u667a\u80fd\u548c\u673a\u5668\u4eba\u4e3a\u5178\u578b\u4ee3\u8868\uff0c\u901a\u8fc7\u7269\u8054\u7f51\u4f20\u611f\u7cfb\u7edf\u5b9e\u73b0\u7269\u7269<\/span>\u76f8\u8fde\uff0c\u4e3a\u5927\u6570\u636e\u63d0\u4f9b\u6e20\u9053\u548c\u6570\u636e\u57fa\u7840\uff1b\u901a\u8fc7\u5bf9\u5927\u6570\u636e\u8fdb\u884c\u5904\u7406\u4e0e\u5206\u6790\uff0c\u4e3a\u4eba\u5de5\u667a\u80fd\u63d0\u4f9b\u7cbe\u51c6\u6570\u636e\u5904\u7406\u624b\u6bb5\u548c\u51b3\u7b56\u4f9d\u636e\uff1b\u901a\u8fc7\u4eba\u5de5\u667a\u80fd\u5b9e\u73b0\u519c\u4e1a\u667a\u80fd\u51b3\u7b56\uff0c\u4e3a\u673a\u5668\u4eba\u65e0\u4eba\u5316<\/span>\u4f5c\u4e1a\u63d0\u4f9b\u6838\u5fc3\u5904\u7406\u624b\u6bb5\uff0c\u56db\u9879\u6280\u672f\u9ad8\u5ea6\u878d\u5408\u6784\u6210\u4e86\u672a\u6765\u65e0\u4eba\u5316\u3001\u7cbe\u51c6\u5316\u3001\u667a\u80fd\u5316\u3001\u751f\u6001\u5316\u519c\u4e1a\u7684\u6838\u5fc3\u652f\u6491\u3002\u519c\u4e1a\u52a8\u690d\u7269\u751f\u7406\u4f20\u611f\u5668\u3001\u52a8\u690d\u7269\u751f\u957f\u4f18\u5316\u6a21\u578b\u3001\u88c5\u5907\u7ba1\u7406\u4e0e\u4f18\u5316\u548c\u667a\u80fd\u673a\u5668\u4eba\u5c06\u662f\u667a\u80fd\u519c\u4e1a\u6280\u672f\u672a\u6765\u7684\u7814\u7a76\u70ed\u70b9\uff1b\u519c\u4e1a\u7269\u8054\u7f51\u5c06\u5411\u5e73\u53f0\u5316\u3001\u6807\u51c6\u5316\u3001\u4f4e\u6210\u672c\u5316\u3001\u9ad8\u53ef\u9760\u6027\u65b9\u5411\u53d1\u5c55\uff0c\u519c\u4e1a\u5927\u6570\u636e\u5c06\u7531\u6570\u636e\u9ad8\u6548\u5b58\u50a8\u5411\u519c\u4e1a\u6570\u636e\u6316\u6398\u548c\u77e5\u8bc6\u53d1\u73b0\u6280\u672f\u65b9\u5411\u53d1\u5c55\uff0c\u4eba\u5de5\u667a\u80fd\u5c06\u5411\u7269\u8054\u7f51<\/span>\u548c\u5927\u6570\u636e\u878d\u5408\u7684\u4eba\u673a\u667a\u80fd\u4fe1\u606f\u8bc6\u522b\u3001\u5206\u7c7b\u548c\u51b3\u7b56\u65b9\u5411\u53d1\u5c55\uff0c\u519c\u4e1a\u673a\u5668\u4eba\u5411\u57fa\u4e8e\u8bed\u8a00\u7406\u89e3\u7684\u5168\u8fc7\u7a0b\u667a\u80fd\u5316\u65b9\u5411\u53d1\u5c55\u3002<\/span><\/p>\n

\uff08\u4e94\uff09\u975e\u4f20\u7edf\u79cd\u690d\u7a7a\u95f4<\/span><\/p>\n

\u76ee\u524d\uff0c\u5168\u7403\u7ea625%<\/span>\u7684\u519c\u5730\u7531\u4e8e\u8fc7\u5ea6\u8015\u4f5c\u3001\u5e72\u65f1\u548c\u6c61\u67d3\u7b49\u539f\u56e0\u4e25\u91cd\u9000\u5316\u3002\u672a\u676530<\/span>\u5e74\uff0c\u5168\u7403\u7cae\u98df\u9700\u6c42\u81f3\u5c11\u589e\u957f60%<\/span>\u3002\u9664\u575a\u5b88\u539f\u8015\u5730\u9762\u79ef\uff0c\u62d3\u5c55\u4f20\u7edf\u8015\u4f5c\u7a7a\u95f4\uff0c\u5b9e\u73b0\u5355\u4f4d\u8d44\u6e90\u9ad8\u6548\u5229\u7528\uff0c\u53d8\u76f8\u589e\u52a0\u79cd\u690d\u7a7a\u95f4\u5916\uff0c\u8fd8\u5e94\u79d1\u5b66\u5f00\u53d1\u5229\u7528\u76d0\u78b1\u5730\u53ca\u8352\u6f20\u7b49\u571f\u5730\u8d44\u6e90\u3002<\/span><\/p>\n

\u76ee\u524d\uff0c\u57ce\u5e02\u519c\u4e1a\u3001\u690d\u7269\u5de5\u5382\u3001\u6c99\u6f20\u79cd\u690d\u548c\u865a\u62df\u79cd\u690d\u7b49\u591a\u9879\u98a0\u8986\u6027\u6280\u672f\u9010\u6b65\u5efa\u7acb\uff0c\u5e76\u6210\u4e3a\u65b0\u7684\u79d1\u6280\u70b9\u548c\u65b0\u7684\u51b2\u7a81\u7206\u53d1\u70b9<\/b>\uff0c\u5bf9\u4e8e\u7f13\u89e3\u5168\u7403\u7cae\u98df\u5b89\u5168\u4e25\u5cfb\u5f62\u52bf\u610f\u4e49\u91cd\u5927\u3002\u672a\u676510~15<\/span>\u5e74\uff0c\u98a0\u8986\u6027\u6280\u672f\u5c06\u6e90\u4e8e\u5404\u9886\u57df\u6280\u672f\u878d\u5408\uff0c\u6216\u57fa\u4e8e\u4f20\u7edf\u6280\u672f\u6df1\u5316\u540e\u7684\u8dc3\u8fc1\u7a81\u53d8\uff0c\u5c06\u63a8\u52a8\u7740\u519c\u4e1a\u7528\u5730\u4e0d\u65ad\u5411\u7a7a\u95f4\uff08\u5305\u62ec\u592a\u7a7a\uff09\u62d3\u5c55\u3002\u5f00\u53d1\u548c\u6539\u826f\u76d0\u78b1\u571f\uff0c\u7efc\u5408\u6cbb\u7406\u517b\u5206\u5931\u8861\u571f\u5730\uff0c\u6df1\u5316\u4f20\u7edf\u8015\u5730\u6280\u672f\uff0c\u5bf9\u4e8e\u589e\u52a0\u540e\u5907\u8015\u5730\uff0c\u4fdd\u969c\u7cae\u98df\u5b89\u5168\u5177\u6709\u91cd\u8981\u610f\u4e49\u3002<\/span><\/p>\n

\u4e09\u3001\u672a\u676510~15<\/span>\u5e74\u5185\u6211\u56fd\u519c\u4e1a\u9886\u57df\u98a0\u8986\u6027\u6280\u672f\u7684\u91cd\u70b9\u65b9\u5411<\/span><\/b><\/p>\n

\uff08\u4e00\uff09\u52a8\u690d\u7269\u9ad8\u6548\u751f\u7269\u80b2\u79cd\u9886\u57df\u91cd\u5927\u98a0\u8986\u6027\u6280\u672f\u65b9\u5411<\/span><\/p>\n

1. <\/span>\u52a8\u690d\u7269\u57fa\u56e0\u7f16\u8f91\u6280\u672f<\/span><\/p>\n

\u7531\u6700\u521d\u7684\u950c\u6307\u6838\u9178<\/span>\u5185\u5207\u9176\uff08ZFN<\/span>\uff09\u53d1\u5c55\u4e3a\u7c7b\u8f6c\u5f55\u6fc0\u6d3b\u56e0\u5b50\u6548\u5e94\u7269\u6838\u9178\u9176\uff08TALEN<\/span>\uff09\u6280\u672f\uff0c\u518d\u5230\u76ee\u524d\u7684CRISPR\/Cas9<\/span>\uff08clustered regulatory
\ninterspaced short palindromic<\/span> repeats
\n\/\u00a0CRISPR-associated protein 9<\/span>\uff09\u7cfb\u7edf\uff0c\u57fa\u56e0\u7f16\u8f91\u6280\u672f\u9776\u5411\u6027\u66f4\u5f3a\u3001\u66f4\u9ad8\u6548\uff0c\u4e5f\u66f4\u5ec9\u4ef7<\/b>\u3002\u5df2\u6210\u529f\u6539\u826f\u4e86\u732a\u3001\u725b\u3001\u7f8a\u548c\u9c7c\u7b49\u6297\u75c5\u6027\u548c\u8089\u8d28\u7b49\uff0c\u4f7f\u6027\u72b6\u6d4b\u91cf\u3001\u53d8\u5f02\u53d1\u73b0\u3001\u53d8\u5f02\u7b5b\u9009\u7b49\u4f20\u7edf\u80b2\u79cd\u8def\u5f84\u66f4\u6539\u4e3a\u76ee\u6807\u6027\u72b6\u7684\u76f4\u63a5\u7f16\u8f91\u548c\u8868\u578b\u786e\u8ba4\uff0c\u80b2\u79cd\u7fa4\u4f53\u5927\u5927\u7f29\u5c0f\uff0c\u80b2\u79cd\u5468\u671f\u5927\u5927\u7f29\u77ed\u3002\u4f46\u6027\u72b6\u57fa\u56e0\u89e3\u6790\u7814\u7a76\u8fd8\u4e0d\u80fd\u63d0\u4f9b\u66f4\u591a\u7684\u53ef\u7f16\u8f91\u57fa\u56e0\u4f4d\u70b9\u3002\u968f\u7740\u529f\u80fd\u57fa\u56e0\u89e3\u6790\u7684\u9010\u6e10\u6df1\u5165\uff0c\u5728\u4eca\u540e 10~15<\/span>\u5e74\u95f4\uff0c\u8be5\u6280\u672f\u5c06\u9010\u6e10\u8d70\u5411\u5927\u89c4\u6a21\u5e94\u7528\uff0c\u5e76\u5bf9\u76f8\u5173\u6027\u72b6\u80b2\u79cd\u4ea7\u751f\u98a0\u8986\u6027\u5f71\u54cd\u3002\u76ee\u524d\uff0c\u8be5\u539f\u521b\u6280\u672f\u6765\u81ea\u56fd\u5916\u3002\u9ad8\u6548\u81ea\u4e3b\u77e5\u8bc6\u4ea7\u6743\u6280\u672f\u5c06\u662f\u6211\u56fd\u7814\u53d1\u7684\u91cd\u70b9\u3002<\/span>\u00a0<\/span><\/p>\n

2. <\/span>\u5bb6\u755c\u5e72\u7ec6\u80de\u80b2\u79cd\u6280\u672f<\/span><\/p>\n

\u5e72\u7ec6\u80de\u80b2\u79cd\u5305\u62ec\u5e72\u7ec6\u80de\u5efa\u7cfb\u57f9\u517b\u3001\u751f\u6b96\u7ec6\u80de\u5b9a\u5411\u5206\u5316\u8bf1\u5bfc\u3001\u4f53\u5916\u53d7\u7cbe\u3001\u57fa\u56e0\u7ec4\u9009\u62e9\u7b49\u6280\u672f\u73af\u8282<\/span><\/b>\uff0c\u76ee\u524d\uff0c\u5bb6\u755c\u5e72\u7ec6\u80de\u5efa\u7cfb\u57f9\u517b\u53ca\u751f\u6b96\u7ec6\u80de\u5b9a\u5411\u5206\u5316\u8bf1\u5bfc\u5c1a\u672a\u53d6\u5f97\u7a81\u7834\u6027\u8fdb\u5c55\uff0c\u4f30\u8ba1\u4eca\u540e 15~20<\/span>\u5e74\uff0c\u4eba\u7c7b\u5c06\u653b\u514b\u4e0a\u8ff0\u6280\u672f\u74f6\u9888\uff0c\u5efa\u7acb\u6210\u719f\u7684\u5e72\u7ec6\u80de\u80b2\u79cd\u6280\u672f\u4f53\u7cfb\u3002\u5e72\u7ec6\u80de\u80b2\u79cd\u5b9e\u73b0\u4e86\u5b9e\u9a8c\u5ba4\u80b2\u79cd\uff0c\u7531\u4e8e\u7ec6\u80de\u9009\u80b2\u73af\u8282\u7684\u5f00\u53d1\uff0c\u4f7f\u9972\u517b\u7684\u6210\u5e74\u52a8\u7269\u79cd\u7fa4\u6570\u91cf\u5927\u5e45\u7f29\u51cf\u3002\u80b2\u79cd\u5468\u671f\u7684\u7f29\u77ed\u3001\u6210\u672c\u7684\u964d\u4f4e\u3001\u573a\u6240\u7684\u6539\u53d8\u7b49\u90fd\u662f\u98a0\u8986\u6027\u7684\u3002\u6211\u56fdiPS<\/span><\/span>\u6280\u672f\u56fd\u9645\u9886\u5148\uff0c\u4f46\u755c\u79bd\u5e72\u7ec6\u80de\u6280\u672f\u5904\u4e8e\u8ddf\u8dd1\u9636\u6bb5\uff0c\u5e94\u52a0\u5feb\u53d1\u5c55\u3002<\/span><\/p>\n

3. <\/span>\u52a8\u690d\u7269\u54c1\u79cd\u5206\u5b50\u8bbe\u8ba1\u6280\u672f<\/span><\/p>\n

\u201c<\/span><\/b>\u54c1\u79cd\u5206\u5b50\u8bbe\u8ba1\u201d<\/span>\u662f\u6307\u4ee5\u5206\u5b50\u8bbe\u8ba1\u7406\u8bba\u4e3a\u6307\u5bfc\uff0c\u7efc\u5408\u8fd0\u7528\u5404\u79cd\u751f\u7269\u4fe1\u606f\u548c\u57fa\u56e0\u64cd\u4f5c\u6280\u672f\uff0c\u4ece\u57fa\u56e0\uff08\u5206\u5b50\uff09\u5230\u6574\u4f53\uff08\u7cfb\u7edf\uff09\u4e0d\u540c\u5c42\u6b21\u5bf9\u76ee\u6807\u6027\u72b6\u8fdb\u884c\u8bbe\u8ba1\u4e0e\u64cd\u4f5c\uff0c\u4ee5\u5b9e\u73b0\u4f18\u826f\u57fa\u56e0\u7684\u6700\u4f73\u914d\u7f6e\uff0c\u57f9\u80b2\u65b0\u54c1\u79cd<\/span><\/b>\u3002\u54c1\u79cd\u5206\u5b50\u8bbe\u8ba1\u6280\u672f\u4f53\u7cfb\u5c06\u63a8\u52a8\u4f20\u7edf\u80b2\u79cd\u5411\u201c<\/span>\u7cbe\u51c6\u80b2\u79cd\u201d<\/span>\u8f6c\u53d8\uff0c\u5927\u5e45\u63d0\u9ad8\u52a8\u690d\u7269\u80b2\u79cd\u6548\u7387\u548c\u6280\u672f\u6c34\u5e73\uff0c\u5f15\u9886\u52a8\u690d\u7269\u80b2\u79cd\u7684\u521b\u65b0\u4e0e\u53d1\u5c55\u3002\u4f30\u8ba1\u901a\u8fc715~20<\/span>\u5e74\u52aa\u529b\uff0c\u5c06\u7a81\u7834\u52a8\u690d\u7269\u591a\u57fa\u56e0\u805a\u5408\u548c\u57fa\u56e0\u7f16\u8f91\u64cd\u4f5c\u6280\u672f\u74f6\u9888\uff0c\u521b\u5efa\u52a8\u690d\u7269\u5206\u5b50\u8bbe\u8ba1\u6280\u672f\u4f53\u7cfb\u3002\u6211\u56fd\u5728\u52a8\u690d\u7269\u591a\u57fa\u56e0\u805a\u5408\u4e0e\u57fa\u56e0\u7f16\u8f91\u9886\u57df\u53d6\u5f97\u4e86\u4e00\u4e9b\u7a81\u7834\u6027\u6210\u679c\uff0c\u4f46\u662f\uff0c\u8feb\u5207\u9700\u8981\u52a0\u5f3a\u5bf9\u52a8\u690d\u7269\u91cd\u8981\u7ecf\u6d4e\u6027\u72b6\u5f62\u6210\u673a\u7406\u8fdb\u884c\u7814\u7a76\uff0c\u4e3a\u5206\u5b50\u8bbe\u8ba1\u9274\u5b9a\u51fa\u66f4\u591a\u7684\u529f\u80fd\u57fa\u56e0\u3002<\/span><\/p>\n

4.<\/span>\u4f53\u5916\u8089\u7c7b\u5408\u6210\u4e0e\u57f9\u517b\u6280\u672f<\/span><\/p>\n

\u901a\u8fc7\u808c\u8089\u5e72\u7ec6\u80de\u57f9\u517b\u4e0e\u5206\u5316\u8bf1\u5bfc\uff0c\u8fdb\u884c\u52a8\u7269\u8089\u54c1\u751f\u4ea7<\/span><\/b>\uff0c\u5df2\u5bf9\u725b\u3001\u9e21\u3001\u9c7c\u7b49\u7269\u79cd\u5b9e\u9a8c\u6210\u529f\uff0c\u8be5\u6280\u672f\u8d8a\u8fc7\u4e86\u9972\u8349\u6599\u751f\u4ea7\u4e0e\u52a0\u5de5\u3001\u52a8\u7269\u9972\u517b\u3001\u52a8\u7269\u5c60\u5bb0\u7b49\u591a\u4e2a\u751f\u4ea7\u73af\u8282\uff0c\u5728\u5b9e\u9a8c\u5ba4\u5373\u53ef\u5b8c\u6210\u52a8\u7269\u8089\u54c1\u751f\u4ea7\u7684\u5168\u8fc7\u7a0b\uff0c\u4e0d\u4f46\u51cf\u5c11\u4e86\u8d44\u6e90\u4e0e\u73af\u5883\u538b\u529b\uff0c\u8fd8\u53ef\u8fdb\u884c\u4ea7\u54c1\u8ba2\u5236\u548c\u6279\u6b21\u5316<\/span>\u751f\u4ea7\u3002\u4f46\u591a\u7ec4\u7ec7\u8054\u5408\u57f9\u517b\u3001\u7ec4\u7ec7\u6df1\u90e8\u4ee3\u8c22\u548c\u5faa\u73af\u6280\u672f\u8fd8\u5728\u7814\u53d1\u4e2d\u3002\u9884\u8ba1\u4eca\u540e15<\/span>\u5e74\u5185\uff0c\u8be5\u6280\u672f\u5c06\u5927\u89c4\u6a21\u5e94\u7528\uff0c\u5e76\u5bf9\u4ea7\u4e1a\u4ea7\u751f\u98a0\u8986\u6027\u5f71\u54cd\u3002\u6211\u56fd\u5b66\u8005\u4ec5\u8fdb\u884c\u808c\u8089\u5e72\u7ec6\u80de\u76f8\u5173\u7814\u7a76\uff0c\u672a\u89c1\u7ec6\u80de\u57f9\u517b\u8089\u9886\u57df<\/span>\u7684\u76f8\u5173\u62a5\u9053\u3002<\/span>\u00a0<\/span><\/p>\n

\uff08\u4e8c\uff09 \u519c\u4e1a\u751f\u7269\u836f\u7269\u4e0e\u751f\u7269\u80a5\u6599\u9886\u57df\u91cd\u5927\u98a0\u8986\u6027\u6280\u672f\u65b9\u5411<\/span><\/p>\n

1. <\/span>\u57fa\u4e8eRNA<\/span>\u5e72\u6270\u6280\u672f\u7684\u57fa\u56e0\u519c\u836f<\/span><\/p>\n

\u5229\u7528RNA<\/span>\u5e72\u6270\u6280\u672f\uff0c\u9632\u6cbb\u75c5\u6bd2\u75c5\u3001\u9632\u9664\u6742\u8349\u3001\u8c03\u8282\u690d\u7269\u91cd\u8981\u9636\u6bb5\u7684\u751f\u957f\uff08\u8131\u53f6\u3001\u6253\u9876\u7b49\uff09\uff0c\u98a0\u8986\u4e86\u4ee5\u5f80\u5316\u5b66\u519c\u836f\u9632\u6cbb\u601d\u8def<\/span><\/b>\u3002\u8be5\u6280\u672f\u74f6\u9888\u662f\u9776\u6807\u57fa\u56e0\u9009\u62e9\u548c\u9ad8\u6548\u7279\u5f02\u8fd0\u8f7d\u4f53\u7cfb\u5f00\u53d1\u3002\u6fb3\u5927\u5229\u4e9a\u548c\u82f1\u56fd\u79d1\u5b66\u5bb6\u5229\u7528\u7eb3\u7c73\u6750\u6599BioClay<\/span><\/span>\u88c5\u8f7ddsRNA<\/span><\/span>\uff0c\u6c89\u9ed8\u540c\u6e90RNA<\/span>\uff0c\u63a7\u5236\u4e86\u70df\u8349\u75c5\u6bd2\u75c5\u3002\u76ee\u524d\u56fd\u9645\u519c\u4e1a\u5316\u5b66\u5de8\u5934\u5df2\u5f00\u59cb\u8fdb\u884c\u76f8\u5173\u6280\u672f\u548c\u4ea7\u54c1\u7684\u7814\u53d1\u3002\u9884\u8ba1\u672a\u67655~10<\/span>\u5e74\u5c06\u6709\u4ea7\u54c1\u8fdb\u5165\u5e94\u7528\uff0c15~30<\/span>\u5e74\u5927\u89c4\u6a21\u5e94\u7528\u3002\u5316\u5b66\u519c\u836f\u5c06\u9010\u6e10\u88ab\u53d6\u4ee3\u3002\u6211\u56fd\u76ee\u524d\u8fd8\u6ca1\u6709\u76f8\u5173\u7684\u7814\u7a76\u62a5\u9053\u3002<\/span><\/p>\n

2. <\/span>\u57fa\u4e8e\u667a\u80fd\u751f\u7269\u6750\u6599\u7684\u519c\u836f\u63a7\u91ca\u6280\u672f<\/span><\/p>\n

\u667a\u80fd\u751f\u7269\u6750\u6599\u662f\u6307\u5229\u7528\u5bf9\u6e29\u5ea6\u3001\u9178\u78b1\u6027\u3001\u6c27\u5316\u8fd8\u539f\u52bf\u7b49\u751f\u7269\u6216\u975e\u751f\u7269\u80c1\u8feb\u4fe1\u53f7\u654f\u611f\u7684\u6750\u6599\uff0c\u5c06\u836f\u7269\u4f20\u9012\u81f3\u9776\u6807\u4f4d\u7f6e\uff0c\u901a\u8fc7\u751f\u7269\u4fe1\u53f7\u53d8\u5316\u7cbe\u786e\u63a7\u5236\u7ed9\u836f\u3002\u9776\u5411\u7ed9\u836f\u548c\u7cbe\u51c6\u65bd\u836f\uff0c\u5bf9\u63d0\u9ad8\u519c\u4f5c\u7269\u6297\u75c5\u6027\u4ee5\u53ca\u53d7\u5230\u5e72\u65f1\u3001\u4f4e\u6e29\u7b49\u975e\u751f\u7269\u9006\u5883\u7684\u9632\u5fa1\u4fdd\u62a4\u610f\u4e49\u91cd\u5927<\/b>\u3002\u6fb3\u5927\u5229\u4e9a\u5bf9\u4ecb<\/span>\u5b54\u4e8c\u6c27\u5316\u7845\u8f7d\u4f53\u8fdb\u884c\u4fee\u9970\uff0c\u5236\u5907\u4e86\u5bf9\u6c27\u5316\u8fd8\u539f\u52bf\u654f\u611f\u7684\u529f\u80fd\u6750\u6599\uff0c\u667a\u80fd\u63a7\u5236\u6c34\u6768\u9178\u91ca\u653e\uff0c\u63d0\u9ad8\u62df\u5357\u82a5<\/span>\u6297\u75c5\u6027\u3002\u76ee\u524d\uff0c\u533b\u836f\u9886\u57df\u6709\u5f88\u591a\u76f8\u5173\u7814\u7a76\u3002\u9884\u8ba1\u672a\u676510~15<\/span>\u5e74\u5c06\u6709\u519c\u836f\u4ea7\u54c1\u51fa\u73b0\u3002\u6211\u56fd\u76ee\u524d\u8fd8\u6ca1\u6709\u76f8\u5173\u7684\u7814\u7a76\u62a5\u9053\u3002<\/span><\/p>\n

3. <\/span>\u4eba\u9020\u75c5\u6bd2\u4f5c\u4e3a\u9884\u9632\u6027\u75ab\u82d7\u6216\u8005\u6cbb\u7597\u6027\u75ab\u82d7<\/span>\u00a0<\/span><\/p>\n

\u5229\u7528\u53cd\u5411\u9057\u4f20\u5b66\u548c\u5408\u6210\u751f\u7269\u5b66\u6280\u672f\uff0c\u6309\u7167\u8ba1\u7b97\u673a\u6a21\u62df\u7a0b\u5e8f\uff0c\u7a81\u53d8\u75c5\u6bd2\u57fa\u56e0\u7ec4\u4e09\u8054\u7801\uff0c\u4eba\u4e3a\u63a7\u5236\u75c5\u6bd2\u590d\u5236\u4ece\u800c\u5c06\u75c5\u6bd2\u76f4\u63a5\u8f6c\u5316\u4e3a\u9884\u9632\u6027\u75ab\u82d7\uff0c\u518d\u7a81\u53d8\u4e3a\u6cbb\u7597\u75c5\u6bd2\u611f\u67d3\u7684\u836f\u7269<\/span><\/b>\u3002\u8fd9\u79cd\u901a\u7528\u65b9\u6cd5\u53ef\u4ee5\u7814\u5236\u4efb\u610f\u81f4\u547d\u6027\u75c5\u6bd2\u7684\u75ab\u82d7\u548c\u6cbb\u7597\u6027\u836f\u7269\uff0c\u5e76\u5f00\u53d1\u5f71\u54cd\u56fd\u9632\u5b89\u5168\u7684\u9884\u9632\u6027\u751f\u5316\u6b66\u5668\u3002\u76ee\u524d\u5317\u4eac\u5927\u5b66\u5df2\u5728\u5b9e\u9a8c\u5ba4\u6c34\u5e73\u7814\u5236\u4e86\u6d41\u611f\u75ab\u82d7\u3002<\/span><\/p>\n

4. <\/span>\u7eb3\u7c73\u4f50\u5242<\/span><\/p>\n

\u7eb3\u7c73\u9897\u7c92\u4e0e\u5fae\u751f\u7269\u76f8\u5f53\uff0c\u80fd\u591f\u66f4\u597d\u5730\u88ab\u6297\u539f\u9012\u5448\u7ec6\u80de\u541e\u566c\uff0c\u8fd8\u53ef\u4ee5\u589e\u52a0\u5c0f\u5206\u5b50\u6297\u539f\u7684\u5c3a\u5bf8\uff0c\u5e76\u5bf9\u5176\u8868\u9762\u8fdb\u884c\u4fee\u9970\uff0c\u589e\u5f3a\u75ab\u82d7\u7684\u673a\u4f53\u514d\u75ab\u5e94\u7b54\u6548\u679c\u3002\u56e0\u6b64\uff0c\u7eb3\u7c73\u9897\u7c92\u6709\u53ef\u80fd\u53d1\u5c55\u6210\u4e3a\u4e00\u7c7b\u65b0\u578b\u7eb3\u7c73\u4f50\u5242<\/b>\u3002\u4f46\u662f\u5176\u4f5c\u7528\u673a\u7406\u53ca\u526f\u4f5c\u7528\u4ecd\u4e0d\u6e05\u695a\uff0c\u5f88\u5c11\u6709\u7eb3\u7c73\u4f50\u5242\u8fdb\u5165\u4e34\u5e8a\u8bd5\u9a8c\u9636\u6bb5\u3002\u6211\u56fd\u5df2\u63d0\u51fa\u4e00\u5957\u6807\u51c6\u5316\u7eb3\u7c73\u6750\u6599\u5b89\u5168\u8bc4\u4ef7\u4f53\u7cfb\uff0c\u4ee5\u8bc4\u4ef7\u7eb3\u7c73\u6750\u6599\u7684\u751f\u7269\u6bd2\u6027\u548c\u7eb3\u7c73\u6548\u5e94\u7b49\uff0c\u6709\u5f85\u89e3\u51b3\u4ee5\u4e0a\u95ee\u9898\u3002<\/span><\/p>\n

5. <\/span>\u4f5c\u7269\u5fae\u751f\u7269\u7ec4\u6280\u672f<\/span><\/p>\n

\u5e94\u7528\u4f5c\u7269\u5fae\u751f\u7269\u7ec4\u6280\u672f\uff0c\u901a\u8fc7\u5bf9\u4e0d\u540c\u4f5c\u7269\u5fae\u751f\u7269\u7ec4\u7684\u9ad8\u901a\u91cf\u5206\u79bb\u548c\u5927\u6570\u636e\u5206\u6790\uff0c\u83b7\u53d6\u5bf9\u4e0d\u540c\u4f5c\u7269\u53d1\u6325\u5404\u79cd\u6709\u76ca\u529f\u80fd\u7684\u5fae\u751f\u7269\u7ec4<\/span><\/b>\uff0c\u901a\u8fc7\u5fae\u751f\u7269\u5408\u6210\u83cc\u7fa4\u5b66\u539f\u7406\u548c\u6280\u672f\uff0c\u7406\u6027\u8bbe\u8ba1\u51fa\u5bf9\u4e0d\u540c\u4f5c\u7269\u53d1\u6325\u7279\u5b9a\u529f\u80fd\u3001\u7ed3\u6784\u7a33\u5b9a\u7684\u7ec4\u5408\u5fae\u751f\u7269\u7fa4\uff0c\u5f00\u53d1\u57fa\u4e8e\u4f5c\u7269\u5fae\u751f\u7269\u7ec4\u6280\u672f\u7684\u9ad8\u6548\u5fae\u751f\u7269\u80a5\u6599\u3002<\/span><\/p>\n

\uff08\u4e09\uff09\u519c\u4e1a\u751f\u7269\u8d28\u5de5\u7a0b\u9886\u57df\u98a0\u8986\u6027\u6280\u672f\u65b9\u5411<\/span><\/p>\n

1. <\/span>\u751f\u7269\u8d28\u6cb9\u3001\u6c14\u8054\u4ea7\u5de5\u7a0b\u6280\u672f<\/span><\/p>\n

\u4ee5\u6728\u8d28\u7ea4\u7ef4\u7d20\u4e3a\u539f\u6599\uff0c\u5927\u89c4\u6a21\u3001\u9ad8\u6548\u7387\u3001\u4f4e\u6210\u672c\u7684\u751f\u4ea7\u751f\u7269\u8d28\u6cb9\u548c\u751f\u7269\u5929\u7136\u6c14\uff0c\u662f\u5bf9\u5e38\u89c4\u5316\u77f3\u8fd0\u8f93\u71c3\u6599\u5e94\u7528\u4f53\u7cfb\u7684\u98a0\u8986<\/span><\/b>\u3002\u901a\u8fc7\u5173\u952e\u5de5\u827a\u3001\u8bbe\u5907\u548c\u9176\u3001\u83cc\u7684\u7a81\u7834\uff0c\u7ed3\u5408\u751f\u7269\u8d28\u8d44\u6e90\u591a\u7ea7\u5229\u7528\u548c\u591a\u4ea7\u54c1\u8054\u5408\u751f\u4ea7\u6a21\u5f0f\uff0c\u4f7f\u751f\u7269\u8d28\u6cb9\u3001\u6c14\u4ea7\u54c1<\/span>\u5177\u6709\u8f83\u5f3a\u7684\u7ecf\u6d4e\u7ade\u4e89\u529b\uff0c\u5927\u89c4\u6a21\u8fdb\u5165\u5e02\u573a\uff0c\u4e2d\u671f\u5185\u5b9e\u73b0\u6bcf\u5e74 1\u00d7<\/span><\/span>108<\/sup><\/span>\u00a0t<\/span>\u751f\u7269\u71c3\u6cb9\u30011\u00d7<\/span><\/span>1011<\/sup>\u00a0m3<\/sup><\/span>\u00a0<\/span>\u751f\u7269\u5929\u7136\u6c14\uff0c\u4e2d\u8fdc\u671f\u7ffb\u756a\u7684\u751f\u4ea7\u80fd\u529b\uff0c\u5b8c\u6210\u5bf9\u8fdb\u53e3\u77f3\u6cb9\u3001\u5929\u7136\u6c14 60% <\/span>\u4ee5\u4e0a\u7684\u66ff\u4ee3\uff0c\u4fdd\u969c\u56fd\u5bb6\u80fd\u6e90\u548c\u91d1\u878d\u5b89\u5168\uff0c\u51cf\u5c11\u79f8\u79c6\u3001\u755c\u79bd\u7caa\u6c61\u7b49\u519c\u6797<\/span>\u5e9f\u5f03\u7269\u6c61\u67d3\u3002\u6211\u56fd\u5728\u8be5\u9886\u57df\u7684\u6280\u672f\u5df2\u7ecf\u63a5\u8fd1\u4ea7\u4e1a\u5316\uff0c\u751f\u7269\u5929\u7136\u6c14\u9886\u57df\u5177\u6709\u4e00\u5b9a\u9886\u5148\u6027\u3002<\/span>\u00a0<\/span><\/p>\n

2. <\/span>\u80fd\u6e90\u4f5c\u7269\u5206\u5b50\u548c\u57fa\u56e0\u8c03\u63a7\u6280\u672f<\/span><\/p>\n

\u98a0\u8986\u4f5c\u7269\u80b2\u79cd\u964d\u4f4e\u4f5c\u7269\u603b\u751f\u7269\u91cf\uff0c\u63d0\u9ad8\u6536\u83b7\u6307\u6570\u7684\u601d\u8def\uff0c\u57f9\u80b2\u9ad8\u5149\u5408\u6548\u7387\u3001\u9ad8\u751f\u7269\u4ea7\u91cf\u548c\u6613\u4e8e\u964d\u89e3\u8f6c\u5316\u5229\u7528\u7684\u65b0\u578b\u4f5c\u7269\u54c1\u79cd<\/span><\/b>\u3002\u901a\u8fc7\u57fa\u56e0\u8c03\u63a7\uff0c\u6539\u53d8\u4f5c\u7269\u5149\u5408\u7279\u6027\u548c\u4f5c\u7269\u6728\u8d28\u7ea4\u7ef4\u7d20\u7ec4\u6210\uff0c\u57f9\u80b2\u9002\u5b9c\u7279\u5b9a\u964d\u89e3\u8f6c\u5316\u9014\u5f84\u7684\u4e13\u7528\u80fd\u6e90\u4f5c\u7269\uff0c\u5982\u9ad8\u542b\u6cb9\u5fae\u85fb\u3001\u6728\u8d28\u7d20\u542b\u91cf\u4f4e\u7684\u6797\u6728\u7b49\uff0c\u4f7f\u603b\u751f\u7269\u91cf\u548c\u8f6c\u5316\u6548\u7387\u5927\u5e45\u63d0\u9ad8\u3001\u8f6c\u5316\u6210\u672c\u5927\u5e45\u964d\u4f4e\u3002\u65b0\u578b\u80fd\u6e90\u4f5c\u7269\u5c06\u6539\u53d8\u519c\u4e1a\u751f\u4ea7\u548c\u4ea7\u4e1a\u94fe\u6a21\u5f0f\uff0c\u63a8\u52a8\u751f\u7269\u8d28\u5de5\u7a0b\u53d1\u5c55\u3002\u6211\u56fd\u5728\u8be5\u9886\u57df\u7684\u6280\u672f\u5904\u4e8e\u8d77\u6b65\u9636\u6bb5\uff0c\u5177\u5907\u8f83\u597d\u7684\u57fa\u56e0\u80b2\u79cd\u6280\u672f\u57fa\u7840\u3002<\/span><\/p>\n

3. <\/span>\u751f\u7269\u8d28\u4e00\u9505\u6cd5\u8f6c\u5316\u5229\u7528\u6280\u672f<\/span><\/p>\n

\u751f\u7269\u8d28\u4e00\u9505\u6cd5\u8f6c\u5316\u5229\u7528\u6280\u672f\u98a0\u8986\u4e86\u5148\u5c06\u751f\u7269\u8d28\u5206\u79bb\u4e3a\u7ea4\u7ef4\u7d20\u3001\u534a\u7ea4\u7ef4\u7d20\u548c\u6728\u8d28\u7d20\uff0c\u7136\u540e\u5206\u522b\u5355\u76f8\u50ac\u5316\u8f6c\u5316\u7684\u8fc7\u7a0b\u7e41\u590d\u3001\u6210\u672c\u9ad8\u3001\u5f97\u7387\u4f4e\u7684\u4f20\u7edf\u6280\u672f\u8def\u7ebf\uff0c\u4f9d\u636e\u7ea4\u7ef4\u7d20\u53ca\u5176\u964d\u89e3\u4ea7\u7269\u7279\u6027\uff0c\u8bbe\u8ba1\u4ee5\u77f3\u58a8\u70ef\u4e3a\u8f7d\u4f53\u7684\u78b3\u57fa\u65e0\u91d1\u5c5e\u50ac\u5316\u3001\u9ad8\u6548\u591a\u76f8\u50ac\u5316\u4f53\u7cfb\uff0c\u5b9e\u73b0\u4ece\u7ea4\u7ef4\u7d20\u7b49\u7cd6\u7c7b\u7269\u8d28\u5230\u751f\u7269\u57fa\u5e73\u53f0\u5316\u5408\u72695-<\/span>\u7f9f\u7532\u57fa\u7ce0\u919b\uff085-HMF<\/span>\uff09\u53ca\u5176\u7cfb\u5217\u544b\u5583\u57fa\u884d\u751f\u7269\u7684\u4e00\u9505\u6cd5\uff08one-pot reaction<\/span>\uff09\u8f6c\u5316<\/b>\uff0c\u6781\u5927\u5730\u7b80\u5316\u64cd\u4f5c\u6b65\u9aa4\u5e76\u63d0\u5347\u6548\u7387\u3001\u964d\u4f4e\u80fd\u8017\u3002\u5927\u5e45\u63d0\u9ad8\u751f\u7269\u8d28\u5229\u7528\u7387\u548c\u6548\u76ca\uff0c\u6574\u4f53\u63a8\u52a8\u4ea7\u4e1a\u53d1\u5c55\u3002\u6211\u56fd\u5728\u8be5\u9886\u57df\u5177\u6709\u4e00\u5b9a\u5148\u8fdb\u6027\u3002<\/span><\/p>\n

4. <\/span>\u6728\u8d28\u7d20\u5929\u7136\u7ed3\u6784\u5229\u7528\u6280\u672f<\/span>\u00a0<\/span><\/p>\n

\u6728\u8d28\u7d20\u8f6c\u5316\u5229\u7528\u6280\u672f\u9650\u5236\u4e86\u6728\u8d28\u7ea4\u7ef4\u7d20\u7684\u5229\u7528\u3002\u6728\u8d28\u7d20\u50ac\u5316\u8f6c\u5316\u53ef\u751f\u6210\u73af\u70f7\u70c3\u5316\u5408\u7269\uff0c\u751f\u4ea7\u9ad8\u6027\u80fd\u822a\u7a7a\u71c3\u6599\uff0c\u7efc\u5408\u6027\u80fd\u53ef\u4ee5<\/span>\u8d85\u8fc7\u77f3\u6cb9\u57fa\u822a\u7a7a\u7164\u6cb9\uff1b\u5229\u7528\u6728\u8d28\u7d20\u5929\u7136\u82ef\u73af\u7ed3\u6784\uff0c\u901a\u8fc7\u7eb3\u7c73\u6280\u672f\u78b3\u5316\u91cd\u6574\uff0c\u5236\u9020\u7eb3\u7c73\u78b3\u7ba1\u6750\u6599\u3001\u5149\u89e3\u6750\u6599\u7b49\u9ad8\u6027\u80fd\u6216\u53ef\u964d\u89e3\u6750\u6599\u3002\u76f4\u63a5\u5229\u7528\u6728\u8d28\u7d20\u5929\u7136\u7ed3\u6784\uff0c\u98a0\u8986\u4e86\u4f20\u7edf\u964d\u89e3\u6728\u8d28\u7d20\u518d\u5229\u7528\u6a21\u5f0f<\/b>\u3002\u8be5\u9886\u57df\u662f\u56fd\u9645\u751f\u7269\u8d28\u5de5\u7a0b\u7684\u65b0\u65b9\u5411\uff0c\u6211\u56fd\u5728\u8be5\u9886\u57df\u5177\u6709\u4e00\u5b9a\u9886\u5148\u6027\u3002<\/span>\u00a0<\/span><\/p>\n

5. <\/span>\u751f\u7269\u8d28\u70ed\u5316\u5b66\u548c\u751f\u7269\u8f6c\u5316\u8026\u5408\u6280\u672f<\/span><\/p>\n

\u5229\u7528\u70ed\u5316\u5b66\u65b9\u6cd5\u964d\u89e3\u751f\u7269\u8d28\u6750\u6599\uff0c\u518d\u5229\u7528\u751f\u7269\u65b9\u6cd5\u5c06\u5c0f\u5206\u5b50\u5316\u5408\u7269\u8f6c\u5316\u6210\u957f\u78b3\u94fe\u5316\u5408\u7269<\/span><\/b>\u3002\u53ef\u5b9e\u73b0\u6e29\u548c\u6761\u4ef6\u4e0b\u9ad8\u8f6c\u5316\u7387\u3001\u4f4e\u6210\u672c\u7684\u751f\u7269\u8d28\u8d44\u6e90\u9ad8\u503c\u5229\u7528\u3002\u4f8b\u5982\uff0c\u91c7\u7528\u78b3\u539f\u6599\uff08CO<\/span>\u3001<\/span>CO2<\/sub><\/span>\u00a0<\/span>\u3001CH4<\/sub>\u00a0<\/span>\u7b49\uff09\u751f\u4ea7\u751f\u7269\u57fa\u80fd\u6e90\u548c\u6750\u6599\u4ea7\u54c1\uff0c\u662f\u5bf9\u77f3\u6cb9\u5316\u5de5\u5927\u5206\u5b50\u88c2\u89e3\u8def\u7ebf\u7684\u98a0\u8986\u3002\u901a\u8fc7\u591a\u79cd\u6280\u672f\u8026\u5408\u5c06\u751f\u7269\u8d28\u539f\u6599\u6db2\u5316\uff0c\u53ef\u4ee5\u514b\u670d\u751f\u7269\u8d28\u677e\u6563\u3001\u5206\u6563\u548c\u80fd\u91cf\u5bc6\u5ea6\u4f4e\u7b49\u95ee\u9898\uff0c\u4f7f\u4e4b\u80fd\u591f\u50cf\u539f\u6cb9\u90a3\u6837\u91c7\u96c6\u3001\u8fd0\u8f93\u548c\u96c6\u4e2d\u52a0\u5de5\u3002\u8be5\u65b9\u5411\u7684\u7a81\u7834\u5c06\u5f7b\u5e95\u6539\u53d8\u6574\u4e2a\u751f\u7269\u8d28\u5de5\u7a0b\u7684\u4ea7\u4e1a\u5f62\u6001\u3002\u8be5\u9886\u57df\u662f\u56fd\u9645\u63a2\u7d22\u7684\u65b0\u65b9\u5411\uff0c\u6211\u56fd\u5728\u8be5\u9886\u57df\u5177\u6709\u4e00\u5b9a\u5148\u8fdb\u6027\u3002<\/span><\/p>\n

\uff08\u56db\uff09\u667a\u80fd\u519c\u4e1a\u9886\u57df\u98a0\u8986\u6027\u6280\u672f\u65b9\u5411<\/span><\/p>\n

1. <\/span>\u519c\u4e1a\u7269\u8054\u7f51\u6280\u672f<\/span><\/p>\n

\u8fd0\u7528\u4f20\u611f\u5668\u7b49\u5404\u7c7b\u611f\u77e5\u6280\u672f\u3001\u5b9e\u65f6\u83b7\u53d6\u519c\u4e1a\u73b0\u573a\u4fe1\u606f\uff0c\u901a\u8fc7\u5404\u7c7b\u7f51\u7edc\u4f20\u8f93\uff0c\u5c06\u4fe1\u606f\u878d\u5408\u5904\u7406\u5e76\u901a\u8fc7\u519c\u4e1a\u4f5c\u4e1a\u7ec8\u7aef\u5b9e\u73b0\u6700\u4f18\u5316\u7684\u7cbe\u51c6\u4f5c\u4e1a\u4e0e\u63a7\u5236<\/span><\/b>\u30022035\u20142050<\/span>\u5e74\uff0c\u519c\u4e1a\u7269\u8054\u7f51\u6280\u672f\u5c06\u5b9e\u73b0\u5168\u9762\u5e94\u7528\u548c\u5f7b\u5e95\u5b9e\u73b0\u519c\u4e1a\u98a0\u8986\u30022035<\/span>\u5e74\u5c06\u8fbe\u52302000<\/span>\u4ebf~3000<\/span>\u4ebf\u5143\u7684\u5e02\u573a\u89c4\u6a21\u3002\u519c\u4e1a\u751f\u4ea7\u8fc7\u7a0b\u5c06\u5b8c\u5168\u53ef\u611f\u77e5\uff0c\u5e76\u5b9e\u73b0\u7cbe\u7ec6\u5316\u3001\u7cbe\u51c6\u5316\u3001\u65e0\u4eba\u5316\u3002\u6211\u56fd\u519c\u4e1a\u7269\u8054\u7f51\u6574\u4f53\u6280\u672f\u5b8c\u5168\u6210\u719f\uff0c\u5546\u4e1a\u5316\u673a\u5236\u4e5f\u5b8c\u5168\u5f62\u6210\u3002<\/span><\/p>\n

2. <\/span>\u519c\u4e1a\u5927\u6570\u636e\u6280\u672f<\/span><\/p>\n

\u5bf9\u5927\u89c4\u6a21\u591a\u6e90\u5f02\u6784\u519c\u4e1a\u6570\u636e\u8fdb\u884c\u91c7\u96c6\u3001\u6e05\u6d17\u3001\u5b58\u50a8\u3001\u6316\u6398\uff0c\u7528\u4e8e\u519c\u4e1a\u667a\u80fd\u4e0e\u7cbe\u51c6\u51b3\u7b56<\/span><\/b>\u30022035<\/span>\u5e74\uff0c\u5927\u6570\u636e\u6280\u672f\u8fdb\u5165\u6210\u719f\u671f\uff0c\u5c06\u4e3a\u519c\u4e1a\u751f\u4ea7\u3001\u7ecf\u8425\u3001\u7ba1\u7406\u548c\u670d\u52a1\u63d0\u4f9b\u5168\u9762\u3001\u51c6\u786e\u3001\u5ba2\u89c2\u7684\u6307\u5bfc\uff0c\u519c\u4e1a\u751f\u4ea7\u6570\u636e\u5316\uff0c\u519c\u6751\u6cbb\u7406\u900f\u660e\u5316\uff0c\u519c\u6c11\u670d\u52a1\u4e2a\u6027\u5316\uff0c\u4ea7\u4e1a\u89c4\u6a21\u5c06\u8fbe2000<\/span>\u4ebf\u5143\u4eba\u6c11\u5e01\u4ee5\u4e0a\u3002<\/span><\/p>\n

3.<\/span>\u519c\u4e1a\u4eba\u5de5\u667a\u80fd\u6280\u672f<\/span>\u00a0<\/span><\/p>\n

\u57fa\u4e8e\u5927\u6570\u636e\u667a\u80fd\u3001\u611f\u77e5\u8ba1\u7b97\u3001\u4eba\u673a\u6df7\u5408\u667a\u80fd\u3001\u7fa4\u4f53\u667a\u80fd\u3001\u81ea\u4e3b\u534f\u540c\u4e0e\u51b3\u7b56\u7b49\u7684\u65b0\u4e00\u4ee3\u4eba\u5de5\u667a\u80fd\u6280\u672f\u5c06\u8986\u76d6\u519c\u4e1a\u751f\u4ea7\u3001\u519c\u6751\u6cbb\u7406\u548c\u519c\u6c11\u751f\u6d3b<\/span><\/b>\u30022035<\/span>\u5e74\u5e02\u573a\u89c4\u6a21\u5c06\u63a5\u8fd12500<\/span>\u4ebf\u5143\u4eba\u6c11\u5e01\u3002\u519c\u4e1a\u751f\u4ea7\u6548\u7387\u3001\u8d44\u6e90\u5229\u7528\u7387\u3001\u571f\u5730\u4ea7\u51fa\u7387\u90fd\u5c06\u6210\u500d\u589e\u52a0\uff0c\u519c\u6751\u6cbb\u7406\u66f4\u52a0\u9ad8\u6548\u900f\u660e\uff0c\u519c\u6c11\u751f\u6d3b\u66f4\u52a0\u4fbf\u5229\u3002\u518d\u8fc710~15<\/span>\u5e74\uff0c\u6211\u56fd\u4eba\u5de5\u667a\u80fd\u5c06\u6df1\u5165\u5230\u519c\u4e1a\u751f\u4ea7\u3001\u519c\u6751\u6cbb\u7406\u548c\u519c\u6c11\u751f\u6d3b\u7684\u5404\u4e2a\u5c42\u9762\uff0c\u5e76\u8fdb\u5165\u66f4\u9ad8\u5c42\u6b21\u3002<\/span><\/p>\n

4. <\/span>\u519c\u4e1a\u673a\u5668\u4eba\u6280\u672f<\/span><\/p>\n

\u5bfc\u822a\u3001\u5b9a\u4f4d\u3001\u8bc6\u522b\u3001\u4f5c\u4e1a\u7b49\u667a\u80fd\u673a\u5668\u4eba\u6280\u672f\u548c\u88c5\u5907\u5c06\u9010\u6e10\u5e94\u7528\u4e8e\u519c\u4e1a\uff0c2035<\/span>\u5e74\u5c06\u8fdb\u5165\u6210\u719f\u671f\uff0c\u5e76\u5927\u89c4\u6a21\u5e94\u7528\u548c\u5b9e\u73b0\u4ea7\u4e1a\u98a0\u8986<\/span><\/b>\uff0c\u8fbe\u5230\u7ea63000<\/span>\u4ebf~5000<\/span>\u4ebf\u5143\u7684\u5e02\u573a\u89c4\u6a21\u3002\u5c06\u6781\u5927\u89e3\u653e\u52b3\u52a8\u751f\u4ea7\u529b\uff0c\u63d0\u9ad8\u52b3\u52a8\u6548\u7387\u3002\u519c\u4e1a\u673a\u5668\u4eba\u6280\u672f\u4e0e\u5de5\u4e1a\u5236\u9020\u3001\u4eba\u5de5\u667a\u80fd\u6280\u672f\u53d1\u5c55\u5bc6\u5207\u76f8\u8fde\uff0c\u968f\u7740\u6211\u56fd\u519c\u6751\u57ce\u9547\u5316\u7684\u8fdb\u4e00\u6b65\u63a8\u8fdb\u548c\u52b3\u52a8\u529b\u8001\u9f84\u5316\u8d8b\u52bf\uff0c\u518d\u8fc710~15<\/span>\u5e74\uff0c\u6211\u56fd\u5c06\u6210\u4e3a\u4e16\u754c\u519c\u4e1a\u673a\u5668\u4eba\u6280\u672f\u7684\u5f15\u9886\u8005\uff0c\u5728\u519c\u4e1a\u8015\u79cd\u3001\u6536\u83b7\u3001\u690d\u4fdd\u4ee5\u53ca\u755c\u79bd\u548c\u6c34\u4ea7\u517b\u6b96\u5404\u4e2a\u73af\u8282\uff0c\u519c\u4e1a\u673a\u5668\u4eba\u5747\u4f1a\u5f97\u5230\u5927\u89c4\u6a21\u5229\u7528\u3002<\/span><\/p>\n

\uff08\u4e94\uff09\u975e\u4f20\u7edf\u79cd\u690d\u7a7a\u95f4\u5229\u7528\u91cd\u5927\u98a0\u8986\u6027\u6280\u672f\u65b9\u5411<\/span><\/p>\n

\uff081<\/span>\uff09\u6839\u636e\u6536\u96c6\u7684\u571f\u58e4\u548c\u690d\u7269\u6570\u636e\uff0c\u5229\u7528\u7535\u8111\u865a\u62df\u79cd\u690d\u519c\u4f5c\u7269<\/b>\uff0c\u52a0\u5feb\u9009\u62e9\u6027\u4f5c\u7269\u80b2\u79cd\u5e76\u5bf9\u8010\u76d0\u54c1\u79cd\u8fdb\u884c\u7b5b\u9009\u548c\u5206\u7c7b\uff0c\u901a\u8fc7\u5206\u5e03\u5f0f\u79cd\u690d\uff0c\u5b9e\u73b0\u519c\u7530\u8282\u6c34\u3001\u6291\u76d0\u5e73\u8861<\/span>\u8c03\u63a7\u548c\u7cbe\u91cf\u704c\u6e89\u3002<\/span><\/p>\n

\uff082<\/span>\uff09\u5f00\u53d1\u9ad8\u6548\u6297\u5835\u585e\u7684\u6839\u533a\u6c34\u76d0\u7cbe\u51c6\u8c03\u63a7\u704c\u6e89\u7cfb\u7edf<\/b>\uff1b\u63ed\u793a\u6697\u7ba1\u6392\u76d0\u5de5\u7a0b\u4e0e\u571f\u58e4\u754c\u9762\u76d0\u5206\u79ef\u805a\u5835\u585e\u673a\u5236\uff1b\u7b5b\u9009\u9ad8\u6548\u6392\u76d0\u6750\u6599\u3001\u4f18\u5316\u9636\u68af\u5f0f\u6697\u7ba1\u6392\u76d0\u5de5\u7a0b\u7ed3\u6784\u4e0e\u5e03\u5c40\uff1b\u4e25\u683c\u9884\u9632\u5730\u4e0b\u6c34\u57cb\u6df1\u8f83\u6d45\u533a\u57df\u571f\u58e4\u6b21\u751f\u76d0\u78b1\u5316\u3002<\/span><\/p>\n

\uff083<\/span>\uff09\u53d1\u5c55\u6570\u5b57\u519c\u4e1a\uff0c\u4ece\u571f\u58e4\u5892\u60c5\u76d1\u6d4b\u5230\u7530\u95f4\u65bd\u80a5\u53ca\u704c\u6e89\u51b3\u7b56<\/b>\uff0c\u4ece\u4f5c\u7269\u75c5\u866b\u5bb3\u76d1\u6d4b\u53ca\u9884\u9632\u5230\u5224\u65ad<\/span>\u6536\u83b7\u65f6\u95f4\u53ca\u91c7\u6458\u7b49\uff0c\u901a\u8fc7\u901a\u4fe1\u7f51\u7edc\u3001\u4eba\u5de5\u667a\u80fd\u3001\u5fae\u578b\u673a\u5668\u4eba\u3001\u65e0\u4eba\u673a\u548c\u4f20\u611f\u5668\u7b49\u5148\u8fdb\u8bbe\u5907\u5b9e\u73b0\u667a\u80fd\u5316\u548c\u4fe1\u606f\u5171\u4eab\u5316\uff0c\u540c\u65f6\u5411\u519c\u6c11\u5b9e\u65f6\u53cd\u9988\u51b3\u7b56\u5b9e\u65bd\u8fc7\u7a0b\u3002<\/span><\/p>\n

\uff084<\/span>\uff09\u7efc\u5408\u519c\u4e1a\u2013<\/span>\u6c34\u4ea7\u517b\u6b96\u7cfb\u7edf\u5b9e\u73b0\u6c34\u7684\u4e8c\u6b21\u5229\u7528\uff0c\u5728\u8fdc\u79bb\u6d77\u5cb8\u7ebf\u7684\u76d0\u78b1\u5730\u533a\u8fdb\u884c\u6c34\u4ea7\u517b\u6b96\uff0c\u5b9e\u73b0\u6c34\u4ea7\u517b\u6b96\u589e\u6c27\u673a\u3001\u6295\u6599\u673a\u3001\u6c34\u6cf5\u7b49\u7535\u5668\u8bbe\u5907\u7ba1\u7406\u667a\u80fd\u5316<\/b>\u30025\u2030~30\u2030<\/span>\u7684\u5fae\u54b8\u6c34\u517b\u6b96\u5bf9\u867e\uff0c\u98a0\u8986\u4e86\u5185\u9646\u5730\u533a\u517b\u6b96\u7bb1\u517b\u6b96\u6d77\u867e\u9700\u5916\u52a0\u6d77\u76d0\u7684\u6280\u672f\u8def\u7ebf\uff0c\u6446\u8131\u4e86\u6d77\u76d0\u6dfb\u52a0\u6210\u672c\u4e0e\u6d77\u5cb8\u7ebf\u8ddd\u79bb\u6b63\u76f8\u5173\u7684\u9650\u5236\u3002<\/span><\/p>\n

\u56db\u3001\u4fc3\u8fdb\u519c\u4e1a\u9886\u57df\u98a0\u8986\u6027\u6280\u672f\u53d1\u5c55\u7684\u5efa\u8bae<\/span><\/b><\/p>\n

\uff08\u4e00\uff09\u52a0\u5feb\u521b\u65b0\u6027\u4eba\u624d\u57f9\u80b2<\/span><\/p>\n

\u957f\u671f\u4ee5\u6765\uff0c\u5bf9\u519c\u4e1a\u91cd\u89c6\u4e0d\u591f\uff0c\u4ee5\u53ca\u519c\u4e1a\u9886\u57df\u57fa\u7840\u6761\u4ef6\u8f83\u5dee\u7b49\u539f\u56e0\uff0c\u5bfc\u81f4\u519c\u4e1a\u9886\u57df\u9ad8\u5c42\u6b21\u521b\u65b0\u578b\u4eba\u624d\u603b\u4f53\u4e0d\u8db3\uff0c\u8d28\u91cf\u6709\u5f85\u63d0\u9ad8\uff0c\u9700\u8981\u52a0\u5927\u57f9\u80b2\u529b\u5ea6\u3002<\/span><\/p>\n

\uff08\u4e8c\uff09\u8bbe\u7acb\u519c\u4e1a\u9886\u57df\u98a0\u8986\u6027\u6280\u672f\u57fa\u7840\u7814\u7a76\u4e13\u9879<\/span><\/p>\n

\u98a0\u8986\u6027\u6280\u672f\u4e3b\u8981\u6e90\u4e8e\u57fa\u7840\u7814\u7a76\u7684\u957f\u671f\u79ef\u7d2f\uff0c\u800c\u6211\u56fd\u519c\u4e1a\u79d1\u7814\u591a\u5e74\u6765\u591a\u5173\u6ce8\u77ed\u671f\u6548\u76ca\u7684\u6280\u672f\u653b\u5173\uff0c\u5bf9\u4e8e\u57fa\u7840\u6027\u3001\u4ea4\u53c9\u6027\u7814\u7a76\u91cd\u89c6\u4e0d\u8db3\uff0c\u9700\u8981\u5efa\u7acb\u957f\u671f\u3001\u7a33\u5b9a\u7684\u7814\u7a76\u4e13\u9879\uff0c\u8fdb\u884c\u957f\u671f\u57f9\u80b2<\/b>\u3002\u6b64\u5916\uff0c\u4e5f\u9700\u8981\u501f\u9274\u519c\u4e1a\u4ea7\u4e1a\u6280\u672f\u4f53\u7cfb\u7684\u7ec4\u7ec7\u65b9\u6cd5\uff0c\u7ec4\u5efa\u4e00\u6279\u957f\u671f\u4ece\u4e8b\u519c\u4e1a\u91cd\u5927\u6218\u7565\u95ee\u9898\u548c\u6218\u7565\u6280\u672f\u7814\u7a76\u7684\u4e13\u95e8\u56e2\u961f\uff0c\u5bf9\u519c\u4e1a\u957f\u8fdc\u53d1\u5c55\u7684\u5173\u952e\u6218\u7565\u95ee\u9898\u548c\u6218\u7565\u65b9\u5411\u8fdb\u884c\u957f\u671f\u7684\u521b\u65b0\u7814\u7a76\u3002<\/span>\u00a0<\/span><\/p>\n

\uff08\u4e09\uff09\u5efa\u7acb\u4ee5\u4f01\u4e1a\u4e3a\u4e3b\u4f53\u7684\u521b\u65b0\u673a\u5236<\/span><\/p>\n

\u5efa\u7acb\u4ee5\u4f01\u4e1a\u4e3a\u4e3b\u4f53\u7684\u521b\u65b0\u673a\u5236\uff0c\u52a0\u5feb\u5f62\u6210\u4ee5\u4f01\u4e1a\u4e3a\u4e3b\u4f53\u7684\u81ea\u4e3b\u521b\u65b0\u5c40\u9762\uff0c\u4ee5\u4fc3\u8fdb\u6280\u672f\u7814\u53d1\u4e0e\u5b9e\u65bd\uff0c\u5e76\u66f4\u5feb\u5730\u5f62\u6210\u4ea7\u4e1a\u89c4\u6a21<\/span><\/b>\u3002\u9700\u8981\u51c6\u786e\u5b9a\u4f4d\u653f\u5e9c\u3001\u4f01\u4e1a\u4e0e\u9ad8\u6821\u95f4\u7684\u5173\u7cfb\uff0c\u65e2\u8981\u66f4\u597d\u6fc0\u53d1\u9ad8\u6821\u79d1\u6280\u521b\u65b0\u80fd\u529b\uff0c\u53c8\u4fdd\u8bc1\u98ce\u9669\u5229\u76ca\u7684\u4e3b\u4f53\u4f01\u4e1a\u80af\u6295\u5165\u3001\u4e50\u4e8e\u6295\u5165\uff0c\u5f62\u6210\u201c<\/span>\u4ea7\u5b66\u7814\u201d<\/span>\u5408\u4f5c\u94fe\u6761\u7684\u826f\u6027\u5faa\u73af\u3002\u4e0d\u91c7\u7528\u884c\u653f\u624b\u6bb5\u5f3a\u5236\u6027\u6d3e\u4efb\u52a1\u3001\u538b\u6307\u6807\uff0c\u5145\u5206\u53d1\u6325\u6280\u672f\u670d\u52a1\u548c\u5e02\u573a\u5f00\u53d1\u7684\u6f5c\u529b\uff0c\u4f7f\u519c\u4e1a\u201c<\/span>\u4ea7\u5b66\u7814\u201d<\/span>\u4e00\u4f53\u5316\u5411\u65b0\u7684\u6c34\u5e73\u53d1\u5c55\u3002\u9700\u8981\u5c3d\u5feb\u5efa\u7acb\u5546\u4e1a\u5316\u6280\u672f\u5e94\u7528\u4f53\u7cfb\uff0c\u5145\u5206\u5c0a\u91cd\u4eba\u624d\u3001\u79d1\u6280\u3001\u8d44\u91d1\u3001\u79cd\u8d28\u7b49\u8d44\u6e90\u81ea\u7531\u6d41\u52a8\u7684\u793e\u4f1a\u5c5e\u6027\uff0c\u79ef\u6781\u6784\u5efa\u4ee5\u5e02\u573a\u4e3a\u4e3b\u5bfc\u7684\u8d44\u6e90\u81ea\u7531\u6d41\u52a8\u4e0e\u4f18\u5316\u914d\u7f6e\u7684\u653f\u7b56\u73af\u5883\uff0c\u786e\u7acb\u4eba\u624d\u548c\u79d1\u6280\u5728\u98a0\u8986\u6027\u6280\u672f\u4ea7\u4e1a\u53d1\u5c55\u4e2d\u7684\u7ecf\u6d4e\u5730\u4f4d\uff0c\u5065\u5168\u4fdd\u62a4\u4e0e\u5229\u7528\u4eba\u624d\u548c\u79d1\u6280\u7684\u653f\u7b56\u673a\u5236<\/span><\/p>\n

\uff08\u56db\uff09\u5efa\u7acb\u9002\u5e94\u4ea7\u4e1a\u53d1\u5c55\u7684\u653f\u7b56\u4f53\u7cfb<\/span><\/p>\n

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